Anticorps Polyclonal de lapin anti-DEK

• Phare
• Validé par KD/KO

DEK Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (2)

N° de cat : 16448-1-AP

Synonymes

D6S231E

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Galerie de données de validation

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  WB analysis using 16448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16448-1-AP

  WB result of DEK antibody (16448-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

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  IP experiment of HeLa using 16448-1-AP

  IP result of anti-DEK (IP:16448-1-AP, 4ug; Detection:16448-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules K-562, cellules T-47D, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Informations sur le produit

16448-1-AP cible DEK dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DEK Protéine recombinante Ag8225
• Nom complet: DEK oncogene
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-50 kDa
• Numéro d’acquisition GenBank: BC035259
• Symbole du gène: DEK
• Identification du gène (NCBI): 7913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transduction. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Publications

Species	Application	Title
mouse	IF	Nature; Maintenance of muscle stem-cell quiescence by microRNA-489.; Authors - Cheung Tom H TH
mouse	IF	Dev Cell; Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.; Authors - Chih-Jen Lin
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Dev Cell; Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit.; Authors - Lu Yue
mouse	WB	Oncogene; Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.; Authors - Y Patel
human,mouse	WB,IF,ChIP	Oncogene; Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.; Authors - Yao-Shun Yang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-27-2025)

Mouse liver lysates were subjected to SDS-PAGE and later immunoblotted with DEK antibody. (1:2500 in 1X TBST). Bands were detected around 52 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Liver tissue

DEK Antibody Western Blot validation (1:2500 dilution) in Liver tissue (Cat no:16448-1-AP)

FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well by IF on PC3 cells fixed in 4% PFA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: PC3 cells

DEK Antibody Immunofluorescence validation (1:25 dilution) in PC3 cells (Cat no:16448-1-AP)

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