Anticorps Polyclonal de lapin anti-Desmin

Desmin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

58

Avis (4)

N° de cat : 16520-1-AP

Synonymes

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin

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Galerie de données de validation

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  WB analysis using 16520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16520-1-AP (Desmin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16520-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16520-1-AP (DES antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16520-1-AP

  IP Result of anti-Desmin (IP:16520-1-AP, 4ug; Detection:16520-1-AP 1:3000) with mouse heart tissue lysate 8000ug.

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  IHC staining of human heart using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16520-1-AP (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16520-1-AP (Desmin antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 16520-1-AP (DES antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16520-1-AP (DES antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16520-1-AP (Desmin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human hysteromyoma using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 16520-1-AP (DES antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human appendix using 16520-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendix using 16520-1-AP (DES antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat heart using 16520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Desmin antibody (16520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 16520-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Desmin antibody (16520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 16520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Desmin antibody (16520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 16520-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 16520-1-AP (Desmin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C2C12 using 16520-1-AP

  1X10^6 C2C12 cell were stained with 0.2ug Desmin antibody (16520-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu d'appendice humain, tissu de côlon humain, tissu de muscle squelettique humain, tissu d'hystéromyome humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules C2C12, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en cytométrie: cellule C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 9 publications below
• IF: See 28 publications below
• IP: See 2 publications below

Informations sur le produit

16520-1-AP cible Desmin dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Desmin Protéine recombinante Ag9742
• Nom complet: desmin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 52-53 kDa
• Numéro d’acquisition GenBank: BC032116
• Symbole du gène: DES
• Identification du gène (NCBI): 1674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Publications

Species	Application	Title
mouse	WB	Theranostics; miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.; Authors - Jing Shi
mouse	IF	Acta Physiol (Oxf); Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.; Authors - Yingying Zhang
human	IF	Oxid Med Cell Longev; Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway.; Authors - Yonghong Xiang
human	WB	J Invest Dermatol; TRAF6 Activates Fibroblasts to Cancer-Associated Fibroblasts through FGF19 in Tumor Microenvironment to Benefit the Malignant Phenotype of Melanoma Cells.; Authors - Yeye Guo
mouse	WB	Aging (Albany NY); Vitellogenin 2 promotes muscle development and stimulates the browning of white fat; Authors - Yilei Li
mouse	WB	Sci Rep; MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a.; Authors - Jinghua Du

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody diluted in 1X PBS, dilution 1:50000, worked well for western blot analysis in mouse liver tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:50000
• Cell Tissue Type: Mouse liver tissue

FH

Brice-Emmanuel (Verified Customer) (09-12-2023)

Perfect if you want to see bandes of degraded desmin. I diluted the antibody 1:5000 in NFDM5%.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Mouse Heart Tissue

Desmin Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:16520-1-AP)

FH

Víctor (Verified Customer) (06-12-2023)

It is a good marker for muscle cells. Indeed, sarcomeric structures can be seen with this antibody. High specificity and low background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Skeletal muscle cells obtained by direct cell reprogramming approaches

Desmin Antibody Immunofluorescence validation (1:500 dilution) in Skeletal muscle cells obtained by direct cell reprogramming approaches (Cat no:16520-1-AP)

FH

Sarah (Verified Customer) (11-16-2019)

This antibody worked well for both WB (1:4000 for 1 hour) and IP (1.6ug Ab/ 500ug Protein)

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: WB- 1:4000, IP- 1.6ug
• Cell Tissue Type: C2C12 Cells

Desmin Antibody Western Blot,Immunoprecipitation, validation (WB- 1:4000, IP- 1.6ug dilution) in C2C12 Cells (Cat no:16520-1-AP)

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