Anticorps Polyclonal de lapin anti-DGCR8 N-terminal

• Phare
• Validé par KD/KO

DGCR8 N-terminal Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25835-1-AP

Synonymes

C22orf12, DGCR8, DGCRK6, Gy1

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Galerie de données de validation

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  WB analysis of A431 cells using 25835-1-AP

  WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.

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  WB analysis of HeLa using 25835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25835-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25835-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25835-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 25835-1-AP

  IP Result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.

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  IF Staining of HeLa using 25835-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431
• Résultats positifs en IP: cellules A431
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

25835-1-AP cible DGCR8 N-terminal dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DGCR8 N-terminal Protéine recombinante Ag22988
• Nom complet: DiGeorge syndrome critical region gene 8
• Masse moléculaire calculée: 773 aa, 86 kDa
• Poids moléculaire observé: 88-100 kDa
• Numéro d’acquisition GenBank: BC078147
• Symbole du gène: DGCR8
• Identification du gène (NCBI): 54487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity; Authors - An Yan
bovine	IF	Proc Natl Acad Sci U S A; Embryonic microRNAs are essential for bovine preimplantation embryo development; Authors - Erika E Paulson