Anticorps Polyclonal de lapin anti-DIS3

• Phare
• Validé par KD/KO

DIS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(17)

Avis (1)

N° de cat : 14689-1-AP

Synonymes

KIAA1008, EXOSC11, EC:3.1.13.-, dis3p, Dis-3

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Galerie de données de validation

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  WB analysis using 14689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 14689-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14689-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14689-1-AP (DIS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 14689-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14689-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14689-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (14689-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14689-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14689-1-AP (DIS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, tissu ovarien de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14689-1-AP cible DIS3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIS3 Protéine recombinante Ag6117
• Nom complet: DIS3 mitotic control homolog (S. cerevisiae)
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC056143
• Symbole du gène: DIS3
• Identification du gène (NCBI): 22894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

Publications

Species	Application	Title
mouse	WB	Nature; A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.; Authors - Chang Hao-Ming HM
human	WB	Mol Cell; DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.; Authors - Sara Macias
human	WB	Sci Adv; Enhanced packaging of U6 small nuclear RNA and splicing-related proteins into extracellular vesicles during HIV infection; Authors - Yiyao Huang
mouse	WB	Nucleic Acids Res; RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.; Authors - Charu Mehta
mouse	IF	Proc Natl Acad Sci U S A; The early macrophage response to pathogens requires dynamic regulation of the nuclear paraspeckle; Authors - Sikandar Azam ; KD Validated
human	WB	EMBO J; The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.; Authors - Yuya Kawabe ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein lysate were loaded on the gel. Primary antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

DIS3 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:14689-1-AP)

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