Anticorps Monoclonal anti-DIS3L2

DIS3L2 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2C9F2

N° de cat : 67623-1-Ig

Synonymes

DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2

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Galerie de données de validation

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  WB analysis using 67623-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 67623-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  FC experiment of Jurkat using 67623-1-Ig

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DIS3L2 Monoclonal antibody (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67623-1-Ig cible DIS3L2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DIS3L2 Protéine recombinante Ag29992
• Nom complet: DIS3 mitotic control homolog (S. cerevisiae)-like 2
• Masse moléculaire calculée: 885 aa, 99 kDa
• Poids moléculaire observé: 65 kDa, 99 kda
• Numéro d’acquisition GenBank: BC030113
• Symbole du gène: DIS3L2
• Identification du gène (NCBI): 129563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential; Authors - Damian M Janecki
human	WB	Cell Rep; Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs; Authors - Claudia Latini

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