Anticorps Polyclonal de lapin anti-DKK1

• Phare
• Validé par KD/KO

DKK1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 21112-1-AP

Synonymes

Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK

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Galerie de données de validation

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  WB analysis using 21112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21112-1-AP (DKK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 21112-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21112-1-AP (DKK1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules K-562
• Résultats positifs en IHC: tissu de gliome humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 6 publications below

Informations sur le produit

21112-1-AP cible DKK1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DKK1 Protéine recombinante Ag15110
• Nom complet: dickkopf homolog 1 (Xenopus laevis)
• Masse moléculaire calculée: 266 aa, 29 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC001539
• Symbole du gène: DKK1
• Identification du gène (NCBI): 22943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Cancer stem cell regulated phenotypic plasticity protects metastasized cancer cells from ferroptosis.; Authors - Mingming Wu ; KD Validated
human	WB	Bioengineered; Exosomes from bone marrow mesenchymal stem cells promoted osteogenic differentiation by delivering miR-196a that targeted Dickkopf-1 to activate Wnt/β-catenin pathway; Authors - Zhi Peng
human	WB	Oncotarget; MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC).; Authors - Bo-Liang Liu
rat	WB	Cells; Sirtuin 6 Overexpression Improves Rotator Cuff Tendon-to-Bone Healing in the Aged; Authors - Young Jae Moon
rat	WB	Osteoporos Int; Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.; Authors - C Liu
human	WB	Biochem Pharmacol; miR-29a-3p-dependent COL3A1 and COL5A1 expression reduction assists sulforaphane to inhibit gastric cancer progression.; Authors - Sichong Han

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