Anticorps Polyclonal de lapin anti-DLX2

DLX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 26244-1-AP

Synonymes

distal less homeobox 2, DLX2, Homeobox protein DLX 2, TES 1, TES1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 26244-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26244-1-AP (DLX2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26244-1-AP (DLX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26244-1-AP (DLX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 26244-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 26244-1-AP (DLX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26244-1-AP cible DLX2 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLX2 Protéine recombinante Ag24663
• Nom complet: distal-less homeobox 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC032558
• Symbole du gène: DLX2
• Identification du gène (NCBI): 1746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLX2, also named as Homeobox protein DLX-2, is a 328 amino acid protein, which contains 1 homeobox DNA-binding domain and belongs to the distal-less homeobox family. DLX2 localizes in the nucleus and is a Phosphoprotein. DLX2 likely to play a regulatory role in the development of the ventral forebrain and may play a role in craniofacial patterning and morphogenesis. The calcualted molecular weight of DLX2 is 34 kDa, but modified DLX2 is about 45-50 kDa. (PMID: 14769946)

Publications

Species	Application	Title
human	WB,IHC,CoIP	iScience; DLX2 promotes osteosarcoma epithelial-mesenchymal transition and doxorubicin resistance by enhancing HOXC8-CDH2 axis; Authors - Boya Zhang
human	IF	PLoS One; Examining the role of the surfactant family member SFTA3 in interneuron specification.; Authors - Christopher Y Chen
mouse	WB	Cell Death Dis; Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.; Authors - Qi Cui
human	IHC	Cancer Lett; Disruption of enhancer-driven S100A14 expression promotes esophageal carcinogenesis; Authors - Xukun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

De (Verified Customer) (08-21-2019)

It has a clear and right-size bind on WB membrane

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human A549, H1650; mouse LLC

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