Anticorps Polyclonal de lapin anti-DMC1

DMC1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 13714-1-AP

Synonymes

DMC1H, HsLim15, LIM15

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Galerie de données de validation

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  WB analysis using 13714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13714-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13714-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13714-1-AP (DMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 13714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMC1 antibody (13714-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DMC1 antibody (13714-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, tissu testiculaire de rat, tissu testiculaire humain
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Informations sur le produit

13714-1-AP cible DMC1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DMC1 Protéine recombinante Ag4653
• Nom complet: DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
• Masse moléculaire calculée: 165 aa, 18 kDa, 38 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC035658
• Symbole du gène: DMC1
• Identification du gène (NCBI): 11144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
mouse	IF	Nucleic Acids Res; MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.; Authors - Yongliang Shang
		Cell Prolif; ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression; Authors - Xu Zhou
mouse	IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang
mouse	IF	Elife; A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.; Authors - Natalia Felipe-Medina
human	IHC	Front Oncol; The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).; Authors - Jennifer Gantchev

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