Anticorps Polyclonal de lapin anti-Dystrophin

• Phare
• Validé par KD/KO

Dystrophin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 12715-1-AP

Synonymes

BMD, CMD3B, DMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, dystrophin

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Galerie de données de validation

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  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12715-1-AP

  WB result of Dystrophin antibody (12715-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dystrophin transfected HepG2 cells.

• 

  IP experiment of mouse brain using 12715-1-AP

  IP result of anti-Dystrophin(IP:12715-1-AP, 4ug; Detection:12715-1-AP 1:20000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 12715-1-AP (Dystrophin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle tissue using 12715-1-AP

  IF result of anti-DMD (12715-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

• 

  IF Staining of rat heart using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Connexin 43 antibody (26980-1-AP, green).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, cerveau de rat, poumon de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris, tissu cardiaque de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 12 publications below
• IP: See 2 publications below

Informations sur le produit

12715-1-AP cible Dystrophin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dystrophin Protéine recombinante Ag3408
• Nom complet: dystrophin
• Masse moléculaire calculée: 3685 aa, 427 kDa
• Poids moléculaire observé: 70 kDa, 430 kDa
• Numéro d’acquisition GenBank: BC028720
• Symbole du gène: DMD
• Identification du gène (NCBI): 1756
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Publications

Species	Application	Title
mouse	WB	Nat Biomed Eng; Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.; Authors - Ningning Shi
mouse	WB,IF	Sci Adv; Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders; Authors - Jihad El Andari
mouse	WB,IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
mouse	IF	Oxid Med Cell Longev; Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.; Authors - Wei Zou
rat	IHC	Oxid Med Cell Longev; Knockdown of Salusin-β Improves Cardiovascular Function in Myocardial Infarction-Induced Chronic Heart Failure Rats.; Authors - Yu Xu
mouse	IF	Nanomedicine; Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination; Authors - JingWen Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lauren (Verified Customer) (08-10-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: stem cell-derived myotubes

Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)

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