Anticorps Polyclonal de lapin anti-DNA2

• Phare
• Validé par KD/KO

DNA2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 18727-1-AP

Synonymes

DNA2, DNA2L, KIAA0083

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Galerie de données de validation

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  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18727-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18727-1-AP

  IP result of anti-DNA2 (IP:18727-1-AP, 4ug; Detection:18727-1-AP 1:300) with mouse liver tissue lysate 4800 ug.

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  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18727-1-AP

  Immunofluorescent analysis of HepG2 cells using 18727-1-AP (DNA2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu de thymus de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18727-1-AP cible DNA2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DNA replication helicase 2 homolog (yeast)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 127 kDa
• Numéro d’acquisition GenBank: NM_001080449
• Symbole du gène: DNA2
• Identification du gène (NCBI): 1763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

Publications

Species	Application	Title
human	WB,IF	Nat Cell Biol; Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.; Authors - Ahmed Emam ; KD Validated
mouse	WB	Genes Dev; Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.; Authors - Shengfeng Xu ; KO Validated
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Cell Cycle; The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.; Authors - Feng-Ling Tsai ; KD Validated
human	WB	Cells; Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.; Authors - Huaping Xiao ; KD Validated
human	IHC	Front Mol Biosci; Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.; Authors - Yongqu Lu