Anticorps Polyclonal de lapin anti-DNAJB6

• Phare
• Validé par KD/KO

DNAJB6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 11707-1-AP

Synonymes

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

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Galerie de données de validation

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  WB analysis using 11707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11707-1-AP (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11707-1-AP

  WB result of DNAJB6 antibody (11707-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells.

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  WB analysis using 11707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11707-1-AP (DNAJB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11707-1-AP

  IP Result of anti-DNAJB6 (IP:11707-1-AP, 4ug; Detection:11707-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11707-1-AP (DNAJB6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11707-1-AP (DNAJB6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11707-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJB6 antibody 11707-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules SH-SY5Y, cellules Y79, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

11707-1-AP cible DNAJB6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB6 Protéine recombinante Ag2258
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 6
• Masse moléculaire calculée: 241 aa, 27 kDa
• Poids moléculaire observé: 27 kDa, 38 kDa
• Numéro d’acquisition GenBank: BC000177
• Symbole du gène: DNAJB6
• Identification du gène (NCBI): 10049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID:20159555).

Publications

Species	Application	Title
human	WB	Neurobiol Dis; DNAJB6 suppresses alpha-synuclein induced pathology in an animal model of Parkinson's disease.; Authors - Sertan Arkan
human,mouse	WB,IHC,IF	J Parkinsons Dis; DNAJB6b is Downregulated in Synucleinopathies.; Authors - Jonas Folke ; KO Validated
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
human	WB,IHC	Mol Carcinog; Overexpression of DNAJB6 promotes colorectal cancer cell invasion through an IQGAP1/ERK-dependent signaling pathway.; Authors - Tong-Tong Zhang ; KD Validated
mouse	WB,IF	STAR Protoc; Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.; Authors - Qianxing Zou
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem