Anticorps Monoclonal anti-DNASE1L3

DNASE1L3 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A4D4

N° de cat : CL594-67041

Synonymes

DNase gamma, DNAS1L3, DHP2, Deoxyribonuclease I-like 3, Deoxyribonuclease gamma

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-67041

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 DNASE1L3 antibody (CL594-67041, Clone: 1A4D4 ) at dilution of 1:200, CL488-Phalloidin (green).

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  FC experiment of HepG2 using CL594-67041

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human DNASE1L3 (CL594-67041, Clone:1A4D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67041 cible DNASE1L3 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNASE1L3 Protéine recombinante Ag28187
• Nom complet: deoxyribonuclease I-like 3
• Masse moléculaire calculée: 305 aa, 36 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC015831
• Symbole du gène: DNASE1L3
• Identification du gène (NCBI): 1776
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes' neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]

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