Anticorps Polyclonal de lapin anti-DRP1 (N-terminal)

DRP1 (N-terminal) Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 26187-1-AP

Synonymes

DLP1, DNM1L, DNM1L,DLP1, DRP1, DRP1 (N-terminal), dynamin 1 like, Dynamin 1 like protein, Dynamin like protein

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Galerie de données de validation

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  WB analysis using 26187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26187-1-AP (DRP1 (N-terminal) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26187-1-AP

  IP Result of anti-DNM1L,DLP1 (IP:26187-1-AP, 4ug; Detection:26187-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  FC experiment of HeLa using 26187-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human DRP1 (N-terminal) (26187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

26187-1-AP cible DRP1 (N-terminal) dans les applications de WB, IP, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRP1 (N-terminal) Protéine recombinante Ag24249
• Nom complet: dynamin 1-like
• Masse moléculaire calculée: 736 aa, 82 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC024590
• Symbole du gène: DNM1L
• Identification du gène (NCBI): 10059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	EMBO Mol Med; High-throughput screening identifies suppressors of mitochondrial fragmentation in OPA1 fibroblasts.; Authors - Emma Cretin
human	WB	Biochem Biophys Res Commun; DUSP1 overexpression attenuates renal tubular mitochondrial dysfunction by restoring Parkin-mediated mitophagy in diabetic nephropathy.; Authors - Chang Lu
human	WB	Asian J Androl; Mannose inhibits the growth of prostate cancer through a mitochondrial mechanism.; Authors - Yu-Lin Deng
	WB	Environ Pollut; Mitochondrial dynamics disruption: Unraveling Dinotefuran's impact on cardiotoxicity; Authors - Jiaping Zhu