Anticorps Polyclonal de lapin anti-DOCK7

DOCK7 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13000-1-AP

Synonymes

dedicator of cytokinesis 7, DOCK7, KIAA1771, ZIR2

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Galerie de données de validation

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  WB analysis using 13000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13000-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13000-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 13000-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13000-1-AP

  IP result of anti-DOCK7(IP:13000-1-AP, 4ug; Detection:13000-1-AP 1:10000) with HeLa cells lysate 1280 ug.

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  IP experiment of HeLa using 13000-1-AP

  IP result of anti-DOCK7 (IP:13000-1-AP, 4ug; Detection:13000-1-AP 1:1000) with HeLa cells lysate 2640 ug.

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  IF Staining of HeLa using 13000-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK7 antibody (13000-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu ovarien de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13000-1-AP cible DOCK7 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOCK7 Protéine recombinante Ag3671
• Nom complet: dedicator of cytokinesis 7
• Masse moléculaire calculée: 2109 aa, 239 kDa
• Poids moléculaire observé: 238-243 kDa
• Numéro d’acquisition GenBank: BC016392
• Symbole du gène: DOCK7
• Identification du gène (NCBI): 85440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events. The DOCK 7 antibody (13000-1-AP) can recognize all the isoforms.

Publications

Species	Application	Title
human	WB	Mol Biol Cell; The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.; Authors - P V Ryder
mouse	WB	Nat Cell Biol; EGFR ligand shifts the role of EGFR from oncogene to tumour suppressor in EGFR-amplified glioblastoma by suppressing invasion through BIN3 upregulation.; Authors - Gao Guo
human	WB,IF,IP,CoIP	Neurobiol Dis; The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors; Authors - Pankaj S Ghate
human	WB	Phytomedicine; Protective effect of ethyl ferulate against hypoxic injury in retinal cells and retinal neovascularization in an oxygen-induced retinopathy model; Authors - Rong Xue