Anticorps Recombinant de lapin anti-DOCK8

DOCK8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230441A2

N° de cat : 83270-2-RR

Synonymes

230441A2, dedicator of cytokinesis 8, MRD2

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Galerie de données de validation

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  WB analysis using 83270-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83270-2-RR (DOCK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 83270-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83270-2-RR (DOCK8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 83270-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DOCK8 antibody (83270-2-RR, Clone: 230441A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of THP-1 using 83270-2-RR

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83270-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DOCK8 Recombinant antibody (83270-2-RR, Clone:230441A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Raji, cellules THP-1
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,
• Résultats positifs en FC (Intra): cellules HeLa, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83270-2-RR cible DOCK8 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DOCK8 Protéine recombinante Ag34585
• Nom complet: dedicator of cytokinesis 8
• Masse moléculaire calculée: 2031 aa, 231 kDa
• Poids moléculaire observé: 239 kDa
• Numéro d’acquisition GenBank: BC019102
• Symbole du gène: DOCK8
• Identification du gène (NCBI): 81704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

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