Anticorps Polyclonal de lapin anti-DPP4/CD26

DPP4/CD26 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 29403-1-AP

Synonymes

CD26, dipeptidyl peptidase 4, ADCP-2, ADCP2, ADCP 2

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Galerie de données de validation

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  WB analysis using 29403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29403-1-AP (DPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 29403-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29403-1-AP (DPP4/CD26 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 29403-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29403-1-AP (DPP4/CD26 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HT-29
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

29403-1-AP cible DPP4/CD26 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DPP4/CD26 Protéine recombinante Ag30160
• Nom complet: dipeptidyl-peptidase 4
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC013329
• Symbole du gène: DPP4
• Identification du gène (NCBI): 1803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; Identification and validation of an immunotherapeutic signature for colon cancer based on the regulatory patterns of ferroptosis and their association with the tumor microenvironment; Authors - Yong Liu
mouse	WB	Front Allergy; High-fat diet and palmitic acid amplify airway type 2 inflammation; Authors - Kris Genelyn Dimasuay
mouse	WB	Biomed Pharmacother; Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa; Authors - Yijing Yang
human	IHC	J Transl Med; Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry; Authors - Zhichao Lai
human,mouse	WB	Cancer Prev Res (Phila); Stool Protein Mass Spectrometry Identifies Biomarkers for Early Detection of Diffuse-type Gastric Cancer; Authors - Chi-Lee C Ho
mouse	IHC	Cancer Med; RBBP4: A novel diagnostic and prognostic biomarker for non-small-cell lung cancer correlated with autophagic cell death; Authors - Yajing Zhan

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