Anticorps Polyclonal de lapin anti-Dermatopontin

• Phare
• Validé par KD/KO

Dermatopontin Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10537-1-AP

Synonymes

Dermatopontin, DPT, TRAMP

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Galerie de données de validation

• 

  WB analysis of mouse skin using 10537-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10537-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10537-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat testis using 10537-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cutané de rat, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu pancréatique humain, tissu cardiaque humain, tissu testiculaire de rat, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

10537-1-AP cible Dermatopontin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dermatopontin Protéine recombinante Ag0801
• Nom complet: dermatopontin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC033736
• Symbole du gène: DPT
• Identification du gène (NCBI): 1805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the growth inhibition activity of TGF-beta1.

Publications

Species	Application	Title
human	IHC	Oncotarget; DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling.; Authors - Ying Fu
human	WB,IHC	Int J Cancer; Dermatopontin: a potential predictor for metastasis of human oral cancer.; Authors - Yamatoji Masanobu M
mouse	IF	Am J Physiol Cell Physiol; Single-cell Transcriptomic Analyses Identifies Extensive Heterogeneity in the Cellular Composition of Mouse Achilles Tendons.; Authors - Andrea J De Micheli
human	IHC	BMC Cancer; QPRT: a potential marker for follicular thyroid carcinoma including minimal invasive variant; a gene expression, RNA and immunohistochemical study.; Authors - Hinsch Nora N
human	WB	Int J Oncol; NRG1 regulates redox homeostasis via NRF2 in papillary thyroid cancer.; Authors - Ting-Ting Zhang ; KD Validated
human	WB,IHC	Ann Transl Med; Identification of the distinctive role of DPT in dilated cardiomyopathy: a study based on bulk and single-cell transcriptomic analysis.; Authors - Yang Lu

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