Anticorps Polyclonal de lapin anti-DRD5

• Phare
• Validé par KD/KO

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 20310-1-AP

Synonymes

D(1B) dopamine receptor, D(5) dopamine receptor, D1beta dopamine receptor, DBDR, Dopamine D5 receptor

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Galerie de données de validation

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  WB analysis using 20310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20310-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 20310-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug DRD5 Polyclonal antibody (20310-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20310-1-AP cible DRD5 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRD5 Protéine recombinante Ag14145
• Nom complet: dopamine receptor D5
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC009748
• Symbole du gène: DRD5
• Identification du gène (NCBI): 1816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

Publications

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
mouse	WB	Mol Cell; Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.; Authors - Yuqing Wu ; KO Validated
mouse	IHC	Int J Mol Sci; Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review.; Authors - Magdalena Kurnik-Łucka
human	WB	ACS Chem Neurosci; Development of a PC12 Cell Based Assay for Screening Catechol-O-methyltransferase Inhibitors; Authors - Gongliang Zhang
human	WB,IF	Cell Oncol (Dordr); Activation of dopamine receptor D1 inhibits glioblastoma tumorigenicity by regulating autophagic activity.; Authors - Kang Yang
mouse	WB	J Neurochem; Validation of the Targeted Metabolomic Pathway in the Hippocampus and Comparative Analysis with the Prefrontal Cortex of Social Defeat Model Mice.; Authors - Ke Xu

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