Anticorps Polyclonal de lapin anti-DRG2

• Phare
• Validé par KD/KO

DRG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 14743-1-AP

Synonymes

Translation factor GTPase DRG2, TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2

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Galerie de données de validation

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  WB analysis using 14743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14743-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14743-1-AP

  IP result of anti-DRG2 (IP:14743-1-AP, 4ug; Detection:14743-1-AP 1:2000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DRG2 antibody (14743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules SMMC-7721, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

14743-1-AP cible DRG2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DRG2 Protéine recombinante Ag6433
• Nom complet: developmentally regulated GTP binding protein 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41-45 kDa
• Numéro d’acquisition GenBank: BC000493
• Symbole du gène: DRG2
• Identification du gène (NCBI): 1819
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.

Publications

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
human	WB	Int J Mol Sci; DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial Dysfunction.; Authors - Anh-Nhung Le ; KO Validated
human	WB,IF,IP	Oxid Med Cell Longev; DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts; Authors - Bing Si Li ; KD Validated
mouse	IF	FASEB J; Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.; Authors - Rebecca Chase
mouse	WB,IHC	J Mol Med (Berl); Role of endosomal RANKL-LGR4 signaling during osteoclast differentiation; Authors - Beom Chang Kim ; KD Validated
human,monkey	WB	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wojciech (Verified Customer) (09-12-2025)

The antibody against DRG2 protein showed a single band at ~40 kDa in T-cells, consistent with the expected molecular weight. The signal was clean with minimal background. No non-specific bands were observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: leukemic T-cells

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