Anticorps Polyclonal de lapin anti-DTX1

• Phare
• Validé par KD/KO

DTX1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18350-1-AP

Synonymes

deltex homolog 1 (Drosophila), Deltex1, DTX1, hDTX1, hDx 1, Protein deltex 1

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Galerie de données de validation

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  WB analysis using 18350-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18350-1-AP (DTX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 18350-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 18350-1-AP (DTX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 18350-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18350-1-AP (DTX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of mouse olfactory epithelium tissue using 18350-1-AP

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of DTX1 antibody (18350-1-AP). Basal cell staining as expected with Sus cell staining on the apical layer. By Dr. Brian Lin (Schwob Lab).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules A375
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'épithélium olfactif de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

18350-1-AP cible DTX1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DTX1 Protéine recombinante Ag13172
• Nom complet: deltex homolog 1 (Drosophila)
• Masse moléculaire calculée: 620 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC048216
• Symbole du gène: DTX1
• Identification du gène (NCBI): 1840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Deltex homolog 1 (E3 ubiquitin-protein ligase DTX1) is a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.DTX1 is conserved from Drosophila, and is expressed in a wide array of tissues, particularly in the central nervous system, testes, and endothelial cells, mainly colocalized with Notch1.It contains a RING-type zinc finger and 2 WWE domains.

Publications

Species	Application	Title
human	WB	Virus Res; Gambogic acid inhibits HBx-mediated hepatitis B virus replication by targeting the DTX1-Notch signaling pathway; Authors - Xu Wen ; KD Validated
human	WB	Cancer Sci; Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.; Authors - Xin-Yu Ding