Anticorps Polyclonal de lapin anti-DUSP7/PYST2

DUSP7/PYST2 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 26910-1-AP

Synonymes

dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2

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Galerie de données de validation

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  WB analysis of rat heart using 26910-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26910-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26910-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

26910-1-AP cible DUSP7/PYST2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DUSP7/PYST2 Protéine recombinante Ag25488
• Nom complet: dual specificity phosphatase 7
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC104880
• Symbole du gène: DUSP7
• Identification du gène (NCBI): 1849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.

Publications

Species	Application	Title
mouse	WB,IHC	Clin Transl Med; Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway; Authors - Chunxiao Ma
human	WB	Mol Cancer; Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer.; Authors - Wan-Xin Peng
human	WB	Br J Pharmacol; L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia.; Authors - Yi-Ping Leng
mouse	WB	Addict Biol; Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory.; Authors - Xiaomeng Qiao
mouse	WB	Genes Cells; Downregulation of DUSP6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2.; Authors - Yuki Kanda