Anticorps Polyclonal de lapin anti-DVL1

• Phare
• Validé par KD/KO

DVL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 27384-1-AP

Synonymes

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Galerie de données de validation

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  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 27384-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27384-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 27384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules MDA-MB-231, cellules NIH/3T3, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

27384-1-AP cible DVL1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DVL1 Protéine recombinante Ag26055
• Nom complet: dishevelled, dsh homolog 1 (Drosophila)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 75 kDa, 73 kDa
• Numéro d’acquisition GenBank: BC050454
• Symbole du gène: DVL1
• Identification du gène (NCBI): 1855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

Publications

Species	Application	Title
human	IHC	Theranostics; Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.; Authors - Junwei Song
mouse	WB	Brain Res Bull; Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.; Authors - Qian Wu
rat	WB	Neural Plast; Lycopene-Loaded Microemulsion Regulates Neurogenesis in Rats with Aβ-Induced Alzheimer's Disease Rats Based on the Wnt/β-catenin Pathway.; Authors - Wen-Jing Ning
mouse	WB	Biol Trace Elem Res; Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components.; Authors - Tingting Lin
rat	WB	Neurosci Lett; Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rats.; Authors - Xiaogang Zhang ; KD Validated
human	IHC	J Obstet Gynaecol; cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.; Authors - Yasushi Yamada