Anticorps Recombinant de lapin anti-DYKDDDDK tag
DYKDDDDK tag Recombinant Antibody for IF/ICC, FC (Intra)
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante
Applications
IF/ICC, FC (Intra)
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240568A11
N° de cat : CL488-80801-2
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules HEK-293 transfectées, |
| Résultats positifs en FC (Intra) | HEK-293T transfected with TDP-43-flag, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-80801-2 cible DYKDDDDK tag dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Recombinant |
| Type | Anticorps |
| Immunogène | DYKDDDDK tag Protéine recombinante Ag2329 |
| Nom complet | DYKDDDDK tag |
| Symbole du gène | |
| Identification du gène (NCBI) | 8 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80801-2 | Download protocol |
| FC protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80801-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH marjolein (Verified Customer) (07-25-2025) | works as it should be
|
FH Fabian (Verified Customer) (04-25-2025) | I compared the antibody in question with the older Antibody (Prod.-No. 80810-1-RR) which is no longer available. In my setting, I tested sample from a purified protein complex which I am working on. I noticed that the older antibody does detect unspecific bands, while the newer antibody in question seems to be more specific. The intensities of the detected signal was comparable and similar. Please find my results attached.
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