Anticorps Recombinant de lapin anti-Decorin

Decorin Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240781E3

N° de cat : 83753-4-RR

Synonymes

DCN, PG40, PG S2, DSPG2, CSCD

---

Galerie de données de validation

• 

  WB analysis using 83753-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 83753-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HEK-293 using 83753-4-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83753-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules ROS1728, tissu de côlon de souris
• Résultats positifs en FC (Intra): cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83753-4-RR cible Decorin dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Decorin Protéine recombinante Ag6275
• Nom complet: decorin
• Masse moléculaire calculée: 359 aa, 40 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC005322
• Symbole du gène: Decorin
• Identification du gène (NCBI): 1634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

{{ptg:RelatedPrimaryAntibodies}}