Anticorps Polyclonal de lapin anti-E2F1

• Phare
• Validé par KD/KO

E2F1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(54)

N° de cat : 12171-1-AP

Synonymes

Retinoblastoma-associated protein 1, RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1

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Galerie de données de validation

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  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 12171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12171-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12171-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12171-1-AP (E2F1 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules HepG2, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 48 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12171-1-AP cible E2F1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: E2F1 Protéine recombinante Ag2813
• Nom complet: E2F transcription factor 1
• Masse moléculaire calculée: 437 aa, 47 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC050369
• Symbole du gène: E2F1
• Identification du gène (NCBI): 1869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Publications

Species	Application	Title
mouse	IHC	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	Cancer Lett; The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.; Authors - Yue Wang ; KD Validated
mouse	IHC	Int J Nanomedicine; E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester); Authors - Jinfeng Xiong
human	IF	Mol Ther; Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.; Authors - Da Zhu
human	WB	Cell Death Dis; SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.; Authors - Jingjing Ma ; KD Validated

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