Anticorps Monoclonal anti-E2F1

• Phare
• Validé par KD/KO

E2F1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(70)

CloneNo.

5D7G8

Avis (2)

N° de cat : 66515-1-Ig

Synonymes

5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1

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Galerie de données de validation

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  WB analysis using 66515-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66515-1-Ig

  WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 59 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below
• ChIP: See 12 publications below

Informations sur le produit

66515-1-Ig cible E2F1 dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: E2F1 Protéine recombinante Ag17363
• Nom complet: E2F transcription factor 1
• Masse moléculaire calculée: 437 aa, 47 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC050369
• Symbole du gène: E2F1
• Identification du gène (NCBI): 1869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

Publications

Species	Application	Title
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB,IF	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
human	WB,IHC,IF,CoIP	Cell Death Dis; Identification of STAM-binding protein as a target for the treatment of gemcitabine resistance pancreatic cancer in a nutrient-poor microenvironment; Authors - Wenming Zhang ; KD Validated
human	WB	Cell Death Dis; ZNF652 exerts a tumor suppressor role in lung cancer by transcriptionally downregulating cyclin D3; Authors - Chunfeng Xie
human	WB	Cell Rep; FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression; Authors - Yanhua Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Umut (Verified Customer) (08-30-2023)

It works very well, even though it is diluted a lot, i.e., 1:2000. The total protein concentration of my samples were around 2 ug/ml before adding 1:4 laemmli buffer and denature, and it is able to capture the target at low exposure times.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U87MG, glioma-derived

E2F1 Antibody Western Blot validation (1:2000 dilution) in U87MG, glioma-derived (Cat no:66515-1-Ig)

FH

Juliane (Verified Customer) (05-11-2023)

The antibody worked fine, but only under using ECL sensitve reagent and high exposure when imaging.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

E2F1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:66515-1-Ig)

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