Anticorps Polyclonal de lapin anti-E2F4

• Phare
• Validé par KD/KO

E2F4 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10923-1-AP

Synonymes

E2F 4, E2F4, Transcription factor E2F4

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Galerie de données de validation

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  WB analysis of HeLa using 10923-1-AP

  WB result of E2F4 antibody (10923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F4 transfected HeLa cells.

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  WB analysis of A431 using 10923-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10923-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10923-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10923-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10923-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10923-1-AP

  IP Result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human lymphoma using 10923-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10923-1-AP (E2F4 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HL-60, cellules Jurkat, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

10923-1-AP cible E2F4 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: E2F4 Protéine recombinante Ag1365
• Nom complet: E2F transcription factor 4, p107/p130-binding
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC033180
• Symbole du gène: E2F4
• Identification du gène (NCBI): 1874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Publications

Species	Application	Title
mouse	WB,ChIP	Protein Cell; Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.; Authors - Yuting Wang
human	WB	Oncogene; Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.; Authors - Wei Huang
mouse	WB,IHC	J Cell Physiol; Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.; Authors - Xiwen Zhang
human	WB	PLoS One; Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.; Authors - Valle Blanca L BL
human	WB	Cell Death Dis; Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.; Authors - Yinghui Wang ; KD Validated
human	WB	Cancers (Basel); Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression.; Authors - Margie N Sutton