Anticorps Polyclonal de lapin anti-EBI3/IL-27B

• Phare
• Validé par KD/KO

EBI3/IL-27B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 12371-1-AP

Synonymes

EBI3, EBV induced gene 3 protein, EBV-induced gene 3 protein, Epstein Barr virus induced 3, Epstein-Barr virus-induced gene 3 protein

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Galerie de données de validation

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  WB analysis using 12371-1-AP

  human placenta tissue was subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 12371-1-AP

  IP result of anti-EBI3 (IP:12371-1-AP, 4ug; Detection:12371-1-AP 1:800) with human placenta tissue lysate 600 ug.

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  IHC staining of human spleen using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 12371-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu splénique de porc
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu splénique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

12371-1-AP cible EBI3/IL-27B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EBI3/IL-27B Protéine recombinante Ag3051
• Nom complet: Epstein-Barr virus induced 3
• Masse moléculaire calculée: 229 aa, 25 kDa
• Poids moléculaire observé: 25-35 kDa
• Numéro d’acquisition GenBank: BC015364
• Symbole du gène: EBI3
• Identification du gène (NCBI): 10148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

Publications

Species	Application	Title
	WB	J Immunol; Role of IL-6-IL-27 Complex in Host Antiviral Immune Response.; Authors - Qi Zuo
human	WB,IF	PLoS One; Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor; Authors - Tong Zhang
		Sci Rep; IL35 modulates HBV-related HCC progression via IL6-STAT3 signaling; Authors - Mingran Zhou
mouse	IF	Pathog Dis; Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.; Authors - Jia-Hui Deng ; KO Validated

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