Anticorps Polyclonal de lapin anti-ECM1

ECM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 11521-1-AP

Synonymes

ECM1, extracellular matrix protein 1, Secretory component p85

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Galerie de données de validation

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  WB analysis of A549 using 11521-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11521-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11521-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11521-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11521-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11521-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11521-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11521-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 11521-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11521-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11521-1-AP (ECM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, cellules HepG2, cellules L02, cellules MCF-7, tissu hépatique de souris, tissu pancréatique de rat, tissu pancréatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 11 publications below
• IF: See 5 publications below

Informations sur le produit

11521-1-AP cible ECM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECM1 Protéine recombinante Ag2084
• Nom complet: extracellular matrix protein 1
• Masse moléculaire calculée: 540 aa, 61 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC023505
• Symbole du gène: ECM1
• Identification du gène (NCBI): 1893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598).

Publications

Species	Application	Title
		Dev Cell; Spatial Control of Primary Ciliogenesis by Subdistal Appendages Alters Sensation-Associated Properties of Cilia.; Authors - Gregory Mazo
human	IF	Oncogene; Extracellular matrix protein 1 promotes cell metastasis and glucose metabolism by inducing integrin β4/FAK/SOX2/HIF-1α signaling pathway in gastric cancer.; Authors - L Gan
human	WB,IHC,IF	J Biol Chem; Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.; Authors - Merregaert Joseph J
human	WB,IHC	Am J Transl Res; miR-23a-5p inhibits cell proliferation and invasion in pancreatic ductal adenocarcinoma by suppressing ECM1 expression.; Authors - Wenjie Huang
human	IHC	Food Chem Toxicol; MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region.; Authors - Lin-Qing Liu
human	IHC,IF	Matrix Biol; ECM1 interacts with fibulin-3 and the beta 3 chain of laminin 332 through its serum albumin subdomain-like 2 domain.; Authors - Sercu S S