Anticorps Polyclonal de lapin anti-Endothelin 1

• Phare
• Validé par KD/KO

Endothelin 1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(27)

N° de cat : 12191-1-AP

Synonymes

EDN1, Big endothelin-1, Endothelin-1, ET 1 Big endothelin 1, ET1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 12191-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12191-1-AP (Endothelin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HUVEC using 12191-1-AP

  1X10^6 HUVEC cells were intracellularly stained with 0.4 ug Anti-Human Endothelin 1 (12191-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu placentaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

12191-1-AP cible Endothelin 1 dans les applications de WB, IHC, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Endothelin 1 Protéine recombinante Ag2847
• Nom complet: endothelin 1
• Masse moléculaire calculée: 212 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC009720
• Symbole du gène: Endothelin 1
• Identification du gène (NCBI): 1906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Publications

Species	Application	Title
mouse	WB	Redox Biol; Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy; Authors - Lijie Ma
human	WB,IP	Cancer Res; Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes.; Authors - Ngoc-Han Ha
human	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
mouse	IF	Redox Biol; Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery.; Authors - Xiaojing Liu
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Cancer Gene Ther; Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.; Authors - Chae Young Hwang ; KD Validated

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