Anticorps Polyclonal de lapin anti-EDNRB

EDNRB Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 20964-1-AP

Synonymes

ABCDS, EDNRB, Endothelin B receptor, endothelin receptor type B, ET B, ET BR, ETB, ETBR, ETRB, HSCR, HSCR2

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Galerie de données de validation

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  WB analysis of HEK-293 using 20964-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 20964-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20964-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20964-1-AP (EDNRB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 20964-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20964-1-AP (EDNRB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 20964-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20964-1-AP (EDNRB Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Raji using 20964-1-AP

  1X10^6 Raji cells were stained with 0.2ug EDNRB antibody (20964-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

20964-1-AP cible EDNRB dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: endothelin receptor type B
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: NM_000115
• Symbole du gène: EDNRB
• Identification du gène (NCBI): 1910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2.; Authors - Masaki Takahashi
mouse	WB	Vascul Pharmacol; Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway.; Authors - Yang Liu
mouse	IF,FC	Pigment Cell Melanoma Res; The immunosuppressive role of Edn3 overexpression in the melanoma microenvironment.; Authors - Juliano T Freitas
mouse	WB	BMC Ophthalmol; Functions of retinal astrocytes and Müller cells in mammalian myopia; Authors - Xuhong Zhang
mouse	WB	J Pharm Pharmacol; Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.; Authors - Zhong-San Zeng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jaouhara (Verified Customer) (12-05-2023)

Immunofluorescence analysis of paraffin-fixed murine chondrocytes using anti-EDNRB (20964-1-AP) and an Alexa 647 secondary antibody. Also validated in flow cytometry and immunohistochemistry.

• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Cell Tissue Type: Chondrocytes

EDNRB Antibody Immunohistochemistry,Immunofluorescence,Flow Cytometry validation ( dilution) in Chondrocytes (Cat no:20964-1-AP)

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