Anticorps Polyclonal de lapin anti-EEA1

• Phare
• Validé par KD/KO

EEA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (2)

N° de cat : 28347-1-AP

Synonymes

Cell and organelle markers, early endosome antigen 1, EEA1, Endosome Marker, MST105, MSTP105, ZFYVE2

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Galerie de données de validation

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  WB analysis of HepG2 using 28347-1-AP

  WB result of EEA1 antibody (28347-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells.

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  WB analysis using 28347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28347-1-AP (EEA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28347-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28347-1-AP (EEA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28347-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28347-1-AP (EEA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28347-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28347-1-AP (EEA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (28347-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 9 publications below

Informations sur le produit

28347-1-AP cible EEA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEA1 Protéine recombinante Ag28814
• Nom complet: early endosome antigen 1
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_003566
• Symbole du gène: EEA1
• Identification du gène (NCBI): 8411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Publications

Species	Application	Title
mouse	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
human	IF	Nucleic Acids Res; Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.; Authors - Huawei Wang
mouse	IF	Front Immunol; Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.; Authors - Yafeng Dou
Human		J Virol; Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis.; Authors - Bo Yang
human	IF	Chemistry; Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction; Authors - Sayan Kundu
human	IF	Cell Death Dis; SorCS3 promotes the internalization of p75NTR to inhibit GBM progression.; Authors - Yanqiu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K. (Verified Customer) (10-26-2023)

This antibody worked nicely for WB at 1:1000 and immunoflurecence at 1:200 dilution.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

FH

Sarah (Verified Customer) (02-09-2023)

Worked well for 1hr IF incubation at RT

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: hct116

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