Anticorps Monoclonal anti-EEA1

• Phare
• Validé par KD/KO

EEA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(17)

CloneNo.

1E11E4

Avis (1)

N° de cat : 68065-1-Ig

Synonymes

1E11E4, Early endosome antigen 1, Endosome-associated protein p162, MST105, MSTP105

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Galerie de données de validation

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  WB analysis of HepG2 using 68065-1-Ig

  WB result of EEA1 antibody (68065-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells.

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  WB analysis using 68065-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68065-1-Ig (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 68065-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68065-1-Ig (EEA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68065-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68065-1-Ig (EEA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68065-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 68065-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EEA1 antibody (68065-1-Ig, Clone: 1E11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Informations sur le produit

68065-1-Ig cible EEA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEA1 Protéine recombinante Ag28814
• Nom complet: early endosome antigen 1
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: NM_003566
• Symbole du gène: EEA1
• Identification du gène (NCBI): 8411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Publications

Species	Application	Title
		EMBO Rep; The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation; Authors - Kaikai Yu
	WB	J Biol Chem; The ROGDI protein mutated in Kohlschutter-Tonz syndrome is a novel subunit of the Rabconnectin-3 complex implicated in V-ATPase assembly; Authors - Samuel R Winkley
human	IF	Life Sci Alliance; ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection; Authors - Melanie Engler
human	IF	J Alzheimers Dis; High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice; Authors - Jian Bao
human	IF	J Virol; Syntaxin-6 restricts SARS-CoV-2 infection by facilitating virus trafficking to autophagosomes; Authors - Hao Sun
pig	IF	J Virol; IFITM proteins are key entry factors for porcine epidemic diarrhea coronavirus; Authors - Lilei Lv

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

great with ecl

• Applications: Western Blot

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