Anticorps Polyclonal de lapin anti-EED

EED Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 16818-1-AP

Synonymes

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

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Galerie de données de validation

• 

  WB analysis of COLO 320 using 16818-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16818-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16818-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16818-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16818-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 16818-1-AP

  IP Result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug.

• 

  IHC staining of human tonsillitis using 16818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16818-1-AP

  Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16818-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16818-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules A431, cellules HeLa, cellules HepG2, cellules K-562, cellules PC-3
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16818-1-AP cible EED dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EED Protéine recombinante Ag10358
• Nom complet: embryonic ectoderm development
• Masse moléculaire calculée: 466 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC068995
• Symbole du gène: EED
• Identification du gène (NCBI): 8726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Publications

Species	Application	Title
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
		J Cell Sci; EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT.; Authors - Shuai-Kun Su
human	WB	Sci Rep; Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.; Authors - Masamitsu N Asaka
human	WB	Cell Signal; Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.; Authors - Zijie Zhang
human	WB	J Cancer; Transcriptional and H3K27ac related genome profiles in oral squamous cell carcinoma cells treated with metformin.; Authors - Shan Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract 20microg/lane. Lane 2-4 transient expression of EZH2.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

EED Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:16818-1-AP)

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