Anticorps Polyclonal de lapin anti-EEF1A1/EEF1A2

• Phare
• Validé par KD/KO

EEF1A1/EEF1A2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 16091-1-AP

Synonymes

EEF1A2, eEF1A-2, EEF1AL, EF-1-alpha-2, Elongation factor 1-alpha 2

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Galerie de données de validation

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  WB analysis using 16091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16091-1-AP (EEF1A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16091-1-AP (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16091-1-AP

  IP result of anti-EEF1A2 (IP:16091-1-AP, 4ug; Detection:16091-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon using 16091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 16091-1-AP (EEF1A2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: human colon, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

16091-1-AP cible EEF1A1/EEF1A2 dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, oyster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF1A1/EEF1A2 Protéine recombinante Ag6875
• Nom complet: eukaryotic translation elongation factor 1 alpha 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC000432
• Symbole du gène: EEF1A2
• Identification du gène (NCBI): 1917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.

Publications

Species	Application	Title
		Nat Commun; EEF1A1 deacetylation enables transcriptional activation of remyelination.; Authors - Mert Duman
mouse,human	WB,IHC,IF	Mol Oncol; SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development.; Authors - Zhiyong Shen
human	WB	Pharmacol Res; Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo.; Authors - Ying Hou
mouse	WB	Sci Rep; Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.; Authors - Faith C J Davies ; KO Validated
human	IF	Br J Cancer; Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression.; Authors - Scaggiante B B
mouse	WB	Hum Mol Genet; Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function.; Authors - Faith C J Davies

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice, very good for western blot and immunofluorescence

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse heart

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