Anticorps Polyclonal de lapin anti-EEF1B2

• Phare
• Validé par KD/KO

EEF1B2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 10483-1-AP

Synonymes

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

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Galerie de données de validation

• 

  WB analysis of HeLa using 10483-1-AP

  WB result of EEF1B2 antibody (10483-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

• 

  WB analysis of PC-3 using 10483-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10483-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10483-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10483-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10483-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10483-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10483-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10483-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10483-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10483-1-AP (EEF1B2 antibody) at dilution of 1:100 (under 25x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules SKOV-3, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10483-1-AP cible EEF1B2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF1B2 Protéine recombinante Ag0742
• Nom complet: eukaryotic translation elongation factor 1 beta 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC004931
• Symbole du gène: EEF1B2
• Identification du gène (NCBI): 1933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.

Publications

Species	Application	Title
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB,IF,IP	Mol Cell Biol; Translational repression by RNA-binding protein TIAR.; Authors - Mazan-Mamczarz Krystyna K
mouse	WB	Front Mol Biosci; Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.; Authors - Taku Kaitsuka
rat	WB	Proteomics; Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.; Authors - Fofana Bourlaye B
human	WB	Amino Acids; Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.; Authors - Yusuke Terui
human,mouse	WB	PLoS One; Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.; Authors - Pitteri Sharon J SJ

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart