Anticorps Monoclonal anti-EEF1D

• Phare
• Validé par KD/KO

EEF1D Monoclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3G4C4

N° de cat : 60085-2-Ig

Synonymes

3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

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Galerie de données de validation

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  WB analysis of MCF-7 using 60085-2-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60085-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 60085-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60085-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60085-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 60085-2-Ig

  IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 60085-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

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  IHC staining of human pancreas cancer using 60085-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50.

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  Affinity and Kinetic Characterization of 60085-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 60085-2-Ig against Human EEF1D were performed. The Affinity Constant is 0.76 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7 transfectées, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60085-2-Ig cible EEF1D dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF1D Protéine recombinante Ag0983
• Nom complet: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC007847
• Symbole du gène: EEF1D
• Identification du gène (NCBI): 1936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB,IF	J Virol; Eukaryotic translation elongation factor 1 delta inhibits the nuclear import of nucleoprotein and PA-PB1 heterodimer of influenza A virus.; Authors - Qingxia Gao ; KO Validated

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