Anticorps Monoclonal anti-EEF1D

• Phare
• Validé par KD/KO

EEF1D Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B1B11

N° de cat : CL488-60085

Synonymes

EF-1-delta, EF1D, EF 1D, EF 1 delta, Antigen NY-CO-4

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-60085

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-60085 (EEF1D antibody), at dilution of 1:100.

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  IF Staining of HeLa using CL488-60085

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-60085 (EEF1D antibody) at dilution of 1:100.

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  FC experiment of MCF-7 using CL488-60085

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using CL488-60085

  1X10^6 HeLa cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human EEF1D (CL488-60085, Clone:3B1B11) (red), or 1 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules MCF-7, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60085 cible EEF1D dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF1D Protéine recombinante Ag0983
• Nom complet: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC007847
• Symbole du gène: EEF1D
• Identification du gène (NCBI): 1936
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

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