Anticorps Polyclonal de lapin anti-eEF2K

• Phare
• Validé par KD/KO

eEF2K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13510-1-AP

Synonymes

eEF-2K, eEF-2 kinase, eEF 2K, eEF 2 kinase, EC:2.7.11.20

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Galerie de données de validation

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  WB analysis of Jurkat using 13510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13510-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13510-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13510-1-AP (EEF2K Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 13510-1-AP

  IP result of anti-eEF2K (IP:13510-1-AP, 4ug; Detection:13510-1-AP 1:500) with PC-3 cells lysate 3200ug.

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  IF Staining of PC-3 using 13510-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13510-1-AP (EEF2K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13510-1-AP cible eEF2K dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: eEF2K Protéine recombinante Ag4432
• Nom complet: eukaryotic elongation factor-2 kinase
• Masse moléculaire calculée: 725 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC032665
• Symbole du gène: EEF2K
• Identification du gène (NCBI): 29904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
human	WB	Oncol Lett; AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.; Authors - Shujuan Tong
rat	WB,IP,CoIP	Cells; SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy.; Authors - Julie Pires Da Silva ; KD Validated
human	WB	Med Oncol; A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells; Authors - Khaled A N Abusharkh
	IHC	JOR Spine; Molecular Subtypes and Immune Microenvironment Characterization of the Annulus Fibrosus in Intervertebral Disc Degeneration: Insights From Translation Factor-Related Gene Analysis; Authors - Sikuan Zheng
		J Mol Med (Berl); FOXM1 plays a role in autophagy by transcriptionally regulating Beclin-1 and LC3 genes in human triple-negative breast cancer cells.; Authors - Zuhal Hamurcu

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