Anticorps Polyclonal de lapin anti-Ephrin A3

• Phare
• Validé par KD/KO

Ephrin A3 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12480-1-AP

Synonymes

EFL 2, EFL2, EFNA3, Ehk1 L, EHK1 ligand, ephrin A3, EPLG3, LERK 3, LERK3

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Galerie de données de validation

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  WB analysis of human brain using 12480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 12480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12480-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12480-1-AP (Ephrin A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12480-1-AP

  IP Result of anti-Ephrin A3 (IP:12480-1-AP, 4ug; Detection:12480-1-AP 1:500) with A549 cells lysate 1040ug.

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  IHC staining of human skeletal muscle using 12480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 12480-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12480-1-AP (Ephrin A3 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 12480-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Ephrin A3 (12480-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12480-1-AP cible Ephrin A3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ephrin A3 Protéine recombinante Ag3188
• Nom complet: ephrin-A3
• Masse moléculaire calculée: 238 aa, 26 kDa
• Poids moléculaire observé: 26-35 kDa
• Numéro d’acquisition GenBank: BC017722
• Symbole du gène: EFNA3
• Identification du gène (NCBI): 1944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Med; EphrinA3 is a key regulator of malignant behaviors and a potential prognostic factor in lung adenocarcinoma.; Authors - Ruzetuoheti Yiminniyaze
human	WB	Biomed Res Int; OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.; Authors - Hui Wang ; KD Validated
human	WB,IF	J Exp Clin Cancer Res; The Ephrin tyrosine kinase a3 (EphA3) is a novel mediator of RAGE-prompted motility of breast cancer cells; Authors - Marianna Talia ; KD Validated
rat	WB	Brain Res Bull; Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats; Authors - Tao Tong