Anticorps Polyclonal de lapin anti-EFTUD2

• Phare
• Validé par KD/KO

EFTUD2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, PLA, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 10208-1-AP

Synonymes

EFTUD2, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD

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Galerie de données de validation

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  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10208-1-AP

  WB result of EFTUD2 antibody (10208-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells.

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  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10208-1-AP (EFTUD2 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10208-1-AP

  IP Result of anti-EFTUD2 (IP:10208-1-AP, 3ug; Detection:10208-1-AP 1:1000) with HeLa cells lysate 4650ug.

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  IP experiment using 10208-1-AP

  IP result of EFTUD2 (10208-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HepG2 using 10208-1-AP

  Immunofluorescent analysis of HepG2 cells, using EFTUD2 antibody 10208-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10208-1-AP cible EFTUD2 dans les applications de WB, PLA, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EFTUD2 Protéine recombinante Ag0279
• Nom complet: elongation factor Tu GTP binding domain containing 2
• Masse moléculaire calculée: 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC002360
• Symbole du gène: EFTUD2
• Identification du gène (NCBI): 9343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Syndromic immune disorder caused by a viable hypomorphic allele of spliceosome component Snrnp40.; Authors - Duanwu Zhang
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
mouse	WB	FASEB J; Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis.; Authors - Jennifer Murray
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	PLA,CoIP	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu