Anticorps Polyclonal de lapin anti-EGF

EGF Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 27141-1-AP

Synonymes

Pro-epidermal growth factor

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Galerie de données de validation

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  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 27141-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 27141-1-AP (EGF antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu cutané humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

27141-1-AP cible EGF dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EGF Protéine recombinante Ag25997
• Nom complet: epidermal growth factor (beta-urogastrone)
• Masse moléculaire calculée: 1207 aa, 134 kDa
• Numéro d’acquisition GenBank: BC093731
• Symbole du gène: EGF
• Identification du gène (NCBI): 1950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal Growth Factor (EGF) is a type of mitogenic factor that stimulates the proliferation of various cells, including epithelial cells and fibroblasts. It is a small peptide composed of 53 amino acids with a molecular weight of approximately 6,000 Daltons. EGF contains three disulfide bonds, which contribute to its stability under acidic and high-temperature conditions. Human EGF is synthesized as transmembrane precursor proteins (1207 amino acids), which are proteolytically cleaved to generate the 54 amino acid mature EGF. EGF plays a crucial role in regulating cell growth, proliferation, and differentiation. When EGF binds to its receptor (EGFR), it activates intracellular signaling pathways that lead to DNA synthesis and cell proliferation. EGF is naturally present in various tissues and body fluids, including saliva, urine, and milk. It is primarily synthesized in the submandibular glands and duodenum.

Publications

Species	Application	Title
human	WB,IHC	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
		Genes (Basel); Evolutionary and Association Analysis of Buffalo FABP Family Genes Reveal Their Potential Role in Milk Performance.; Authors - Tingzhu Ye
human	WB	J Ethnopharmacol; Proteomics analysis of tumor exosomes reveals vital pathways of Jinfukang inhibiting circulating tumor cells metastasis in lung cancer.; Authors - Zu-Jun Que
human	IHC	Int J Exp Pathol; Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma; Authors - Chao Zeng
human	WB	Evid Based Complement Alternat Med; Evodiamine Inhibits Gastric Cancer Cell Proliferation via PTEN-Mediated EGF/PI3K Signaling Pathway.; Authors - Ruichuang Yang
human	WB	ACS Appl Mater Interfaces; Paper/PMMA Hybrid 3D Cell Culture Microfluidic Platform for the Study of Cellular Crosstalk; Authors - Kin Fong Lei

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