Anticorps Polyclonal de lapin anti-Pikachurin

Pikachurin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 14578-1-AP

Synonymes

EGFLAM, EGF-like, fibronectin type-III and laminin G-like domain-containing protein, AGRNL, Agrin-like protein, AGRINL

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Galerie de données de validation

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  WB analysis of human placenta using 14578-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14578-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14578-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 14578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Pikachurin antibody (14578-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 14578-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Pikachurin antibody (14578-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules PC-3, cellules SH-SY5Y
• Résultats positifs en IF-P: tissu oculaire de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

14578-1-AP cible Pikachurin dans les applications de WB, IF/ICC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pikachurin Protéine recombinante Ag5986
• Nom complet: EGF-like, fibronectin type III and laminin G domains
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC033177
• Symbole du gène: Pikachurin
• Identification du gène (NCBI): 133584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Publications

Species	Application	Title
mouse	IF	Stem Cells Dev; Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation.; Authors - Chen Liang
human	WB,CoIP	Int Immunopharmacol; EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma; Authors - Kaishu Li
mouse	IF	iScience; The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells; Authors - James F Striebel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (07-01-2025)

This antibody works nicely on cryosections, antigen retrieval is a must (Citrate Buffer, pH 6). Does not work on paraffin-embedded tissues.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 200
• Cell Tissue Type: Adult mouse retina

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