Anticorps Monoclonal anti-Pikachurin

Pikachurin Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1C6D12

Avis (1)

N° de cat : 66232-1-Ig

Synonymes

EGFLAM, 1C6D12, Agrin like protein, AGRINL, Agrin-like protein

---

Galerie de données de validation

• 

  WB analysis of human retina using 66232-1-Ig

  human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66232-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of rat eye using 66232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Y79 using 66232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu rétinien humain, cellules PC-3
• Résultats positifs en IF-P: tissu oculaire de rat,
• Résultats positifs en IF/ICC: cellules Y79,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66232-1-Ig cible Pikachurin dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Pikachurin Protéine recombinante Ag6362
• Nom complet: EGF-like, fibronectin type III and laminin G domains
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 111 kDa, 55-60 kDa
• Numéro d’acquisition GenBank: BC033177
• Symbole du gène: Pikachurin
• Identification du gène (NCBI): 133584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma; Authors - Kaishu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Federico (Verified Customer) (02-18-2020)

The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 2000x
• Cell Tissue Type: overexpressing protein samples from HEK293 cells, conditioned media from Y79 cells

{{ptg:RelatedPrimaryAntibodies}}