Anticorps Polyclonal de lapin anti-EGFR (C-terminal)

EGFR (C-terminal) Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 51071-2-AP

Synonymes

EGFR, EGFR (C-terminal), ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

---

Galerie de données de validation

• 

  WB analysis of A431 using 51071-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51071-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51071-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 51071-2-AP

  IP Result of anti-EGFR (C-terminal) (IP:51071-2-AP, 5ug; Detection:51071-2-AP 1:500) with A431 cells lysate 3000ug.

• 

  IF Staining of A431 using 51071-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules L02
• Résultats positifs en IP: cellules A431
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

51071-2-AP cible EGFR (C-terminal) dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Masse moléculaire calculée: 1210 aa, 134 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC094761
• Symbole du gène: EGFR
• Identification du gène (NCBI): 1956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.

Publications

Species	Application	Title
human	WB	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
mouse	WB	Cell Chem Biol; Discovery of a covalent inhibitor of heat shock protein 90 with antitumor activity that blocks the co-chaperone binding via C-terminal modification.; Authors - Li Li
human	WB	Front Pharmacol; Exploring the pharmacological mechanisms of icaritin against nasopharyngeal carcinoma via network pharmacology and experimental validation; Authors - Minglu Liu
human	WB,IF	Int J Mol Sci; JAC4 Inhibits EGFR-Driven Lung Adenocarcinoma Growth and Metastasis through CTBP1-Mediated JWA/AMPK/NEDD4L/EGFR Axis; Authors - Kun Ding
human	WB	Biochem Biophys Res Commun; A Wnt/β-catenin signaling inhibitor, IMU1003, suppresses the emergence of osimertinib-resistant colonies from gefitinib-resistant non-small cell lung cancer cells; Authors - Hiroshi Katagiri
human	WB	Steroids; The location of estrogen receptor variant ER-α36 is associated with the invasion of glioblastoma; Authors - Hongyan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Anna (Verified Customer) (02-27-2024)

Very good working antibodies in wetsren blot method

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: GC-1 spg cell culture

EGFR (C-terminal) Antibody Western Blot validation (1:2000 dilution) in GC-1 spg cell culture (Cat no:51071-2-AP)