Anticorps Polyclonal de lapin anti-EHD1

• Phare
• Validé par KD/KO

EHD1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 24657-1-AP

Synonymes

EH domain containing 1, EH domain containing protein 1, EHD1, H PAST, HPAST1, PAST, PAST homolog 1, PAST1, Testilin

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Galerie de données de validation

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  WB analysis using 24657-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24657-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 24657-1-AP

  IP result of anti-EHD1 (IP:24657-1-AP, 4ug; Detection:24657-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HeLa, tissu cérébral de souris, tissu pulmonaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

24657-1-AP cible EHD1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHD1 Protéine recombinante Ag18400
• Nom complet: EH-domain containing 1
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC104799
• Symbole du gène: EHD1
• Identification du gène (NCBI): 10938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	WB	Sci Rep; Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells.; Authors - Shaarvari Bhat ; KD Validated
human	WB,IHC,IF	BMC Cancer; PMID: 27411790; Authors - Jing Gao ; KD Validated
human	WB,IF	Biochem Biophys Res Commun; A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis.; Authors - Hiroki Yukimoto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on thrombosis study. I use this for mouse platelet Western blot and it works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse platelet

FH

Juan (Verified Customer) (05-02-2019)

It works well with a single band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000-1:2000
• Cell Tissue Type: Hela cells