Anticorps Polyclonal de lapin anti-EHD1

• Phare
• Validé par KD/KO

EHD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (3)

N° de cat : 24657-1-AP

Synonymes

Testilin, PAST1, PAST, EH domain-containing protein 1, EH domain containing 1

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Galerie de données de validation

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  WB analysis using 24657-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24657-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24657-1-AP (EHD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24657-1-AP

  IP result of anti-EHD1 (IP:24657-1-AP, 4ug; Detection:24657-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human stomach cancer using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 24657-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24657-1-AP (EHD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HeLa, tissu cérébral de souris, tissu pulmonaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, human intrahepatic cholangiocarcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

24657-1-AP cible EHD1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHD1 Protéine recombinante Ag18400
• Nom complet: EH-domain containing 1
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC104799
• Symbole du gène: EHD1
• Identification du gène (NCBI): 10938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EHD1 (Eps15 Homology Domain Containing 1) is a protein that plays a crucial role in endocytic recycling, which is the process by which cells recycle their membrane components. It is one of four paralogs in mammals (EHD1-4) and is involved in several membrane trafficking pathways. EHD1 is particularly well-studied and is known to regulate the recycling of various cell surface receptors back to the cell surface after they have been endocytosed. This process is essential for maintaining the proper distribution of receptors and other membrane proteins, which in turn affects cellular signaling and function. EHD1 has been implicated in the regulation of the transferrin receptor (TfR), major histocompatibility complex (MHC) class I proteins, β1 integrins, and other receptors. It has also been shown to interact with Rab11-FIP2 and is localized to peripheral endosomes, suggesting a role in the transport of receptors from early endosomes to the endocytic recycling compartment (ERC). Furthermore, EHD1 has been linked to dynein motors that drive transport from early endosomes to the ERC via a complex including MICAL-L1 and the collapsin response mediator protein-2 (Crmp2).

Publications

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	WB	Sci Rep; Rab35 and its effectors promote formation of tunneling nanotubes in neuronal cells.; Authors - Shaarvari Bhat ; KD Validated
human	WB,IHC,IF	BMC Cancer; PMID: 27411790; Authors - Jing Gao ; KD Validated
human	WB,IF	Biochem Biophys Res Commun; A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis.; Authors - Hiroki Yukimoto
		J Extracell Vesicles; Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells; Authors - Sara Busatto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olga (Verified Customer) (09-12-2025)

The blot is quite clear but a bit confusing that there are two bands in mouse cell lines and tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse spleen, heart, lung, liver, forebrain; mouse primary cerebellar granule neurons; mouse cell lines Neuro2A and mHypo

EHD1 Antibody Western Blot validation (1:1000 dilution) in Mouse spleen, heart, lung, liver, forebrain; mouse primary cerebellar granule neurons; mouse cell lines Neuro2A and mHypo (Cat no:24657-1-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on thrombosis study. I use this for mouse platelet Western blot and it works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse platelet

FH

Juan (Verified Customer) (05-02-2019)

It works well with a single band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000-1:2000
• Cell Tissue Type: Hela cells

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