Anticorps Polyclonal de lapin anti-EHD2

• Phare
• Validé par KD/KO

EHD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (2)

N° de cat : 11440-1-AP

Synonymes

PAST2, PAST homolog 2, EH domain-containing protein 2, EH domain containing protein 2, EH domain containing 2

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Galerie de données de validation

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  WB analysis using 11440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11440-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11440-1-AP (EHD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 11440-1-AP

  IP result of anti-EHD2 (IP:11440-1-AP, 4ug; Detection:11440-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11440-1-AP (EHD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11440-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11440-1-AP (EHD2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'œsophage humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11440-1-AP cible EHD2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHD2 Protéine recombinante Ag2002
• Nom complet: EH-domain containing 2
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC014445
• Symbole du gène: EHD2
• Identification du gène (NCBI): 30846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EH domain-containing protein 2 (EHD2) is a member of the EHD protein family. It is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. EHD2 is also a nuclear-cytoplasmic shuttle protein, acting as a corepressor of transcription to inhibit gene transcription when it enters the cell nucleus. EHD2 is a dynamin-related ATPase that plays a key role in regulating a series of biological processes. As a crucial regulator of endocytic transport, it is involved in the formation and maintenance of endosomal tubules and vesicles, which are vital for intracellular transport of proteins and other substances. EHD2 has been implicated in several human cancers and has potential as a predictive biomarker of chemotherapy efficacy.

Publications

Species	Application	Title
human	WB	Am J Cancer Res; Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.; Authors - Junqiang Li ; KD Validated
human	IF	PLoS One; Super-resolution analysis of PACSIN2 and EHD2 at caveolae.; Authors - Tamako Nishimura
human	IHC	Cancer Invest; EHD2 Overexpression Suppresses the Proliferation, Migration, and Invasion in Human Colon Cancer.; Authors - Chengqi Guan
	WB	Cancer Invest; Identification of Differentially Expressed Proteins of Normal and Cancerous Human Colorectal Tissues by Liquid Chromatograph-Mass Spectrometer Based on iTRAQ Approach.; Authors - Jingjing Lv
mouse	WB	Nature; Post-translational control of beige fat biogenesis by PRDM16 stabilization; Authors - Qiang Wang
human,pig	WB,IF	Emerg Microbes Infect; Identification of cepharanthine as an effective inhibitor of African swine fever virus replication; Authors - Chuanxiang Qi ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laura (Verified Customer) (01-27-2022)

It works well for western blot with different bands but one specific around 65 kDa. It also works well for immunofluorescence of cells.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000WB 1/100IF
• Cell Tissue Type: MOVAS (Mouse Primary vascular aortic smooth muscle cells) and RPE-1 (Human retinal pigment epithelial-1)

FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs sample for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs

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