Anticorps Polyclonal de lapin anti-EHD4

EHD4 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 11382-2-AP

Synonymes

EH domain containing 4, EH domain containing protein 4, EHD4, HCA10, HCA11, PAST homolog 4, PAST4

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Galerie de données de validation

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  WB analysis using 11382-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11382-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11382-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11382-2-AP (EHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 11382-2-AP

  Immunofluorescent analysis of HepG2 cells, using EHD4 antibody 11382-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293T, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

11382-2-AP cible EHD4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EHD4 Protéine recombinante Ag1942
• Nom complet: EH-domain containing 4
• Masse moléculaire calculée: 541 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC006287
• Symbole du gène: EHD4
• Identification du gène (NCBI): 30844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

H-domain containing 4, also known as EHD4, belongs to the EHD protein family. Northern blot analysis detected expression of 4 mRNA species of approximately 2.0, 2.7, 3.2, and 3.6 kb. All of the transcripts are highly expressed in pancreas, and the 2.7- and 3.6-kb transcripts are highly expressed in heart. Only weak expression is found in other tissues.

Publications

Species	Application	Title
human	WB	EBioMedicine; EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.; Authors - Quan Zhou
mouse	WB,IHC	J Am Soc Nephrol; Reduced Glomerular Filtration in Diabetes is Attributable to Loss of Density and Increased Resistance of Glomerular Endothelial Cell Fenestrations.; Authors - Natalie Finch
mouse	WB	Curr Biol; EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress.; Authors - Ivana Yeow
mouse	WB	Life Sci; EHD3 positively regulated by NR5A1 participates in testosterone synthesis via endocytosis.; Authors - Lingling Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

I works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T