Anticorps Polyclonal de lapin anti-EIF1AD

• Phare
• Validé par KD/KO

EIF1AD Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20528-1-AP

Synonymes

EIF1AD, Haponin

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Galerie de données de validation

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  WB analysis of K-562 using 20528-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20528-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 20528-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20528-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, cellules HEK-293, cellules HL-60, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20528-1-AP cible EIF1AD dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF1AD Protéine recombinante Ag14390
• Nom complet: eukaryotic translation initiation factor 1A domain containing
• Masse moléculaire calculée: 165 aa, 19 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC005131
• Symbole du gène: EIF1AD
• Identification du gène (NCBI): 84285
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; Structural basis for the final steps of human 40S ribosome maturation.; Authors - Michael Ameismeier ; KD Validated
human	WB	Elife; The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.; Authors - Laura Plassart
human	WB	Elife; Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36.; Authors - Jasmin van den Heuvel
human	WB	Life Sci Alliance; CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau; Authors - Juan Carlos Polanco

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