Anticorps Polyclonal de lapin anti-EIF2AK1

• Phare
• Validé par KD/KO

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(20)

N° de cat : 20499-1-AP

Synonymes

EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor

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Galerie de données de validation

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  WB analysis using 20499-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20499-1-AP

  WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 20499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20499-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20499-1-AP cible EIF2AK1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EIF2AK1 Protéine recombinante Ag14236
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 1
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC006524
• Symbole du gène: EIF2AK1
• Identification du gène (NCBI): 27102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

Publications

Species	Application	Title
	WB	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
human	WB,CoIP	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
human	WB	Science; Human NLRP1 is a sensor for double-stranded RNA.; Authors - Stefan Bauernfried
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KD Validated
human	WB	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty ; KD Validated
human	WB	Mol Cell; Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.; Authors - Jack Chun-Chieh Hsu

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